Correction for multiple comparison over many ROIs?

What is the best correction for multiple comparisons?

The most conservative of corrections, the Bonferroni correction is also perhaps the most straightforward in its approach. Simply divide α by the number of tests (m). However, with many tests, α* will become very small. This reduces power, which means that we are very unlikely to make any true discoveries.

Do I need to correct for multiple comparisons?

Some statisticians recommend never correcting for multiple comparisons while analyzing data (1,2). Instead report all of the individual P values and confidence intervals, and make it clear that no mathematical correction was made for multiple comparisons. This approach requires that all comparisons be reported.

Why is it important to correct for multiple comparisons in the analysis of fMRI data?

In fMRI research, the goal of correcting for multiple comparisons is to identify areas of activity that reflect true effects, and thus would be expected to replicate in future studies.

What is the major problem of multiple comparisons?

In statistics, the multiple comparisons, multiplicity or multiple testing problem occurs when one considers a set of statistical inferences simultaneously or infers a subset of parameters selected based on the observed values. The more inferences are made, the more likely erroneous inferences become.

What are multiple comparison methods?

Multiple comparison methods (MCMs) are designed to investigate differences between specific pairs of means or linear combinations of means. This provides the information that is of most use to the researcher.

How do you correct p values for multiple comparisons?

The simplest way to adjust your P values is to use the conservative Bonferroni correction method which multiplies the raw P values by the number of tests m (i.e. length of the vector P_values).

Is Bonferroni correction necessary?

When conducting multiple analyses on the same dependent variable, the chance of committing a Type I error increases, thus increasing the likelihood of coming about a significant result by pure chance. To correct for this, or protect from Type I error, a Bonferroni correction is conducted.

Why is multiple testing a problem?

What is the Multiple Testing Problem? If you run a hypothesis test, there’s a small chance (usually about 5%) that you’ll get a bogus significant result. If you run thousands of tests, then the number of false alarms increases dramatically.

How does multiple testing correction work?

Perhaps the simplest and most widely used method of multiple testing correction is the Bonferroni adjustment. If a significance threshold of α is used, but n separate tests are performed, then the Bonferroni adjustment deems a score significant only if the corresponding P-value is ≤α/n.

How can the chance of committing a Type I error be reduced when performing multiple comparisons?

A Type I error is when we reject a true null hypothesis. Lower values of α make it harder to reject the null hypothesis, so choosing lower values for α can reduce the probability of a Type I error.

How do benjamini Hochberg correction?

Calculate each individual p-value’s Benjamini-Hochberg critical value, using the formula (i/m)Q, where:

  1. i = the individual p-value’s rank,
  2. m = total number of tests,
  3. Q = the false discovery rate (a percentage, chosen by you).


How do I use FDR correction in Excel?


First we need to sort the p values in ascending. Order. Next we will assign rank for the samples. You just have to drag 2 until the very.

What is Benjamini Hochberg method?

The Benjamini–Hochberg method controls the False Discovery Rate (FDR) using sequential modified Bonferroni correction for multiple hypothesis testing.

What is FDR q-value?

q-value is a widely used statistical method for estimating false discovery rate (FDR), which is a conventional significance measure in the analysis of genome-wide expression data. q-value is a random variable and it may underestimate FDR in practice.

What is FDR correction?

The false discovery rate (FDR) is a statistical approach used in multiple hypothesis testing to correct for multiple comparisons. It is typically used in high-throughput experiments in order to correct for random events that falsely appear significant.

What is FDR adjusted p-value?

The FDR is the ratio of the number of false positive results to the number of total positive test results: a p-value of 0.05 implies that 5% of all tests will result in false positives. An FDR-adjusted p-value (also called q-value) of 0.05 indicates that 5% of significant tests will result in false positives.

Is p-value false positive rate?

A positive is a significant result, i.e. the p-value is less than your cut off value, normally 0.05. A false positive is when you get a significant difference where, in reality, none exists. As I mentioned above, the p-value is the chance that this data could occur given no difference actually exists.

How do you protect against false positives?

  1. Avoid excessive testing (think before data exploration)
  2. Keep track of number of tests conducted and report all tests.
  3. Bonferroni correction, false-discovery rate or emphasize preliminary nature of findings.
  4. Average effect sizes across conceptually similar tests.
  5. What is the difference between false discovery rate and p-value?

    The false discovery rate is the complement of the positive predictive value (PPV) which is the probability that, when you get a ‘significant’ result there is actually a real effect. So, for example, if the false discovery rate is 70%, the PPV is 30%.

    Is 0.039 statistically significant?

    The P value of 0.039 is not compelling evidence by itself. The vaccine does not have a proven track record of significant results. The confidence interval indicates that that the estimated effect size is both small and imprecise.

    What does p-value of 0.05 mean?

    The smaller the p-value, the stronger the evidence that you should reject the null hypothesis. A p-value less than 0.05 (typically ≤ 0.05) is statistically significant. It indicates strong evidence against the null hypothesis, as there is less than a 5% probability the null is correct (and the results are random).

    Why do we use 0.05 level of significance?

    For example, a significance level of 0.05 indicates a 5% risk of concluding that a difference exists when there is no actual difference. Lower significance levels indicate that you require stronger evidence before you will reject the null hypothesis.

    Is p-value of 0.1 significant?

    The smaller the p-value, the stronger the evidence for rejecting the H0. This leads to the guidelines of p < 0.001 indicating very strong evidence against H0, p < 0.01 strong evidence, p < 0.05 moderate evidence, p < 0.1 weak evidence or a trend, and p ≥ 0.1 indicating insufficient evidence[1].

    What is the difference between 0.01 and 0.05 level of significance?

    Different levels of cutoff trade off countervailing effects. Lower levels – such as 0.01 instead of 0.05 – are stricter, and increase confidence in the determination of significance, but run an increased risk of failing to reject a false null hypothesis.

    How do you test the hypothesis at 0.05 level of significance?

    To graph a significance level of 0.05, we need to shade the 5% of the distribution that is furthest away from the null hypothesis. In the graph above, the two shaded areas are equidistant from the null hypothesis value and each area has a probability of 0.025, for a total of 0.05.